Myosin, Fast Skeletal, Recombinant, Human, aa1-169, His-Tag (A1 Catalytic, A2 Catalytic, Alkali Myosin Light Chain 1, Alkali Myosin Light Chain 3, DKFZp779C0757, Fast Skeletal Myosin Alkali Light Chain 1, Fast Skeletal Myosin Light Chain 2, MGC13450, MLC1F, MLC3F, Myosin Light Chain 1 Alkali Skeletal Fast, MYL1, MYLPF, Myosin Light Chain 1 Skeletal Muscle Isoform, Myosin Light Chain 2, Myosin Light Chain 3 Skeletal Muscle Isoform, Myosin Regulatory Light Chain 2, MRLC2)
Catalog No : USB-M9848-05R
564.38€
0.00€
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| Product name | Myosin, Fast Skeletal, Recombinant, Human, aa1-169, His-Tag (A1 Catalytic, A2 Catalytic, Alkali Myosin Light Chain 1, Alkali Myosin Light Chain 3, DKFZp779C0757, Fast Skeletal Myosin Alkali Light Chain 1, Fast Skeletal Myosin Light Chain 2, MGC13450, MLC1F, MLC3F, Myosin Light Chain 1 Alkali Skeletal Fast, MYL1, MYLPF, Myosin Light Chain 1 Skeletal Muscle Isoform, Myosin Light Chain 2, Myosin Light Chain 3 Skeletal Muscle Isoform, Myosin Regulatory Light Chain 2, MRLC2) | ||
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| Catalog No | USB-M9848-05R | ||
| Supplier’s Catalog No | M9848-05R | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E.coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 21.2 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Purified | ||
| Purity | ~85% (SDS-PAGE) | ||
| Form | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8, 1mM DTT, 100mM sodium chloride, 10% glycerol. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~85% (SDS-PAGE) | ||
| Description | Myosin regulatory light chains, including MRCL3, MYLPF and MYL9, regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases the actin-activated myosin ATPase activity, thereby regulating the contractile activity. MYLPF is critically important for fast and slow skeletal muscle development. Purified by using conventional chromatography techniques. Source: Recombinant corresponding to aa1-169 of human MYLPF, fused to His-tag at N-terminus, expressed in E.coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MAPKRAKRRT VEGGSSSVFS MFDQTQIQEF KEAFTVIDQN RDGIIDKEDL RDTFAAMGRL NVKNEELDAM MKEASGPINF TVFLTMFGEK LKGADPEDVI TGAFKVLDPE GKGTIKKKFL EELLTTQCDR FSQEEIKNMW AAFPPDVGGN VDYKNICYVI THGDAKDQE Molecular Weight: ~21.2kD (189aa), confirmed by MALDI-TOF Enzyme Activity: ATPase activity not determined. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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