MYL9, Recombinant, Human, aa1-172, His-Tag (Myosin regulatory light polypeptide 9, LC20, MLC2, MRLC1, MYRL2)

Catalog No : USB-351380
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Product name MYL9, Recombinant, Human, aa1-172, His-Tag (Myosin regulatory light polypeptide 9, LC20, MLC2, MRLC1, MYRL2)
Catalog No USB-351380
Supplier’s Catalog No 351380
Supplier US Biologicals
Source antigen Recombinant, human from E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 21.9
Storage -20°C/-70°C
Other names
Grade Purified
Purity ~90% (SDS-PAGE)
Form Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 1mM DTT, 10% glycerol.
Reactivity life 12 months
Note For reserch purpose only
Purity ~90% (SDS-PAGE)
Description MYL9, also known as MLC2, is one of the numerous regulatory myosin light chains. Regulatory myosin light chains regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase(MLCK). Phosphorylation of regulatory myosin light chains is catalyzed by MLCK in the presence of calcium and calmodulin and it increases the actin-activated myosin ATPase activity, thereby regulates the contractile activity. Source: Recombinant protein corresponding to aa1-172 from human MYL9, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~21.9kD (192aa) AA Sequence: MGSSHHHHHH SSGLVPRGSH MSSKRAKAKT TKKRPQRATS NVFAMFDQSQ IQEFKEAFNM IDQNRDGFID KEDLHDMLAS LGKNPTDEYL EGMMSEAPGP INFTMFLTMF GEKLNGTDPE DVIRNAFACF DEEASGFIHE DHLRELLTTM GDRFTDEEVD EMYREAPIDK KGNFNYVEFT RILKHGAKDK DD Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer