Neurofilament, Light Polypeptide (NEFL) Recombinant, Mouse
Catalog No : USB-156062
401.16€
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| Product name | Neurofilament, Light Polypeptide (NEFL) Recombinant, Mouse | ||
|---|---|---|---|
| Catalog No | USB-156062 | ||
| Supplier’s Catalog No | 156062 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 12.6 | ||
| Storage | -70°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥95%. Endotoxin: ≤1EU/ug (LAL) | ||
| Form | Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.01% sodium azide. Reconstitute with sterile PBS, pH 7.3. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥95%. Endotoxin: ≤1EU/ug (LAL) | ||
| Description | Source: Recombinant protein corresponding to aa139-234 (P08551) with a N-Terminal His-Tag from mouse NEFL expressed in E. coli Predicted Molecular Weight: 12.6kD Predicted Isoelectric Point: 4.8 Amino Acid Sequence: MGHHHHHHSGSEF-YEQEIRDLRL AAEDATNEKQ ALQGEREGLE ETLRNLQARY EEEVLSREDA EGRLMEARKG ADEAALARAE LEKRIDSLMD EIAFLKKVHE EEIAEL Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Included Protein Molecular Weight Marker: 168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed. MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kD Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions. | ||
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