Imugex

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Categories: Peptides & Proteins / Proteins

Product name			Neuronal Specific Enolase (NSE), Recombinant, Human
Catalog No			USB-N2175-02Q
Supplier’s Catalog No			N2175-02Q
Supplier			US Biologicals
Source antigen			Human, Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			-20°C
Other names
Grade			Highly Purified
Purity			≥95% (SDS-PAGE)
Form			Supplied as a liquid in 50% glycerol.
Reactivity life			6 months
Note			For reserch purpose only
Purity			≥95% (SDS-PAGE)
Description			Recombinant NSE comprises a 433 amino acid fragment (2-434) corresponding to processed mature NSE protein and is expressed in E. coli with an amino- terminal hexahistidine tag. NSE is an isoform of the glycolytic enzyme enolase and is expressed primarily in neurons, in normal and neoplastic neuroendocrine cells. NSE is a highly soluble cytoplasmic protein that is readily secreted into the CSF and serum following tissue damage. NSE shows neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons and binds in a calcium-dependent manner to cultured neocortical neurons promoting cell survival. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.