NSE, Recombinant, Human (Gamma-enolase, 2-phospho-D-glycerate Hydro-lyase, Enolase 2, Neural Enolase, Neuron-specific Enolase, ENO2)

Catalog No : USB-N2175-01R
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Product name NSE, Recombinant, Human (Gamma-enolase, 2-phospho-D-glycerate Hydro-lyase, Enolase 2, Neural Enolase, Neuron-specific Enolase, ENO2)
Catalog No USB-N2175-01R
Supplier’s Catalog No N2175-01R
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -20°C
Other names
Grade Purified
Purity Purified (SDS-PAGE)
Form Supplied as a liquid in 25mM Tris-HCl, pH6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue, 2.5% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity Purified (SDS-PAGE)
Description NSE is the gamma/gamma isoform of the glycolytic enzyme enolase and is expressed primarily in neurons, in normal and neoplastic neuroendocrine cells . NSE is a highly soluble cytoplasmic protein that is readily secreted into the CSF and serum following tissue damage. NSE shows neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons and binds in a calcium-dependent manner to cultured neocortical neurons promoting cell survival. Source: Recombinant corresponding to aa2-434 from mature human NSE fused to 6x His-tag at N-terminal, expressed in E. coli. Applications: Suitable for use in Western Blot as a control. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.