Imugex

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Categories: Peptides & Proteins / Proteins

Product name			Neuronal Specific Enolase, Recombinant, Human, His-Tag, aa2-434 (NSE, Enolase 2)
Catalog No			USB-209114
Supplier’s Catalog No			209114
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			-20°C
Other names
Grade			Highly Purified
Purity			~95% (SDS-PAGE). Single band on Western Blot.
Form			Supplied as a liquid in 25mM Tris-HCl, pH 6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue, 2.5% glycerol.
Reactivity life			12 months
Note			For reserch purpose only
Purity			~95% (SDS-PAGE). Single band on Western Blot.
Description			NSE is the gamma-gamma isoform of the glycolytic enzyme enolase and is expressed primarily in neurons, in normal and neoplastic neuroendocrine cells. NSE is a highly soluble cytoplasmic protein that is readily secreted into the CSF and serum following tissue damage. NSE shows neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons and binds in a calcium-dependent manner to cultured neocortical neurons promoting cell survival. Source: Recombinant protein corresponding to human NSE, containing 433aa (aa2-434), fused to his-tag at N-terminal, expressed in E. coli. Applications: Suitable for use directly as Positive Control for Western Blot, ELISA, Immunoprecipitation and other immunological experiments. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.