Neuron Specific Enolase, Recombinant, His-Tag, aa1-434 (NSE)

Catalog No : USB-298544
495.42€
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Product name Neuron Specific Enolase, Recombinant, His-Tag, aa1-434 (NSE)
Catalog No USB-298544
Supplier’s Catalog No 298544
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (SDS-PAGE 12%)
Form Supplied as a liquid in 20mM sodium phosphate, pH 7.4, 150mM sodium chloride, 50% glycerol.
Reactivity life 12 months
Note For reserch purpose only
Purity ~95% (SDS-PAGE 12%)
Description NSE is the gamma/gamma isoform of the glycolytic enzyme enolase and is expressed primarily in neurons, in normal and neoplastic neuroendocrine cells. NSE is a highly soluble cytoplasmic protein that is readily secreted into the CSF and serum following tissue damage. NSE shows neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons and binds in a calcium-dependent manner to cultured neocortical neurons promoting cell survival. Source: Recombinant protein corresponding to aa1-434 of NSE, fused to 6His-tag expressed in E. coli. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.