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Categories: Peptides & Proteins / Proteins

Product name			SNAP 25, Recombinant, C. elegans (Synaptosomal-Associated Protein)
Catalog No			USB-S1014-74
Supplier’s Catalog No			S1014-74
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			23

Storage			-20°C
Other names
Grade			Highly Purified
Purity			≥ 95% by SDS PAGE; Purified by using conventional chromatography techniques.
Form			Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 50mM sodium chloride, 5mM DTT, 1mM EDTA and 10% glycerol.
Reactivity life			6 months
Note			For reserch purpose only
Purity			≥ 95% by SDS PAGE; Purified by using conventional chromatography techniques.
Description			SNAP-25 is a presynaptic plasma membrane protein essential for synaptic vesicle fusion and neurotransmitter release. The membrane proteins SNAP 25, synaptobrevin and syntaxin make up the core complex (SNARE) essential for docking and holding synaptic vesicles at the pre synaptic membrane. SNAP-25 gene was amplified by PCR from C. elegans and cloned into an E. coli expression vector. Molecular Weight: 23kD (207 amino acids) Sequence : MSGDDDIPEG/ LEAINLKMNA/ TTDDSLESTR/ RMLALCEESK/ EAGIKTLVML/ DDQGEQLERC/ EGALDTINQD/ MKEAEDHLKG/ MEKCCGLCVL/ PWNKTDDFEK/ TEFAKAWKKD/ DDGGVISDQP/ RITVGDSSMG/ PQGGYITKIT/ NDAREDEMDE/ NVQQVSTMVG/ NLRNMAIDMS/ TEVSNQNRQL/ DRIHDKAQSN/ EVRVESANKR/ AKNLITK Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.