SNAP 25, Recombinant, C. elegans (Synaptosomal-Associated Protein 25)

Catalog No : USB-S1014-71C
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Product name SNAP 25, Recombinant, C. elegans (Synaptosomal-Associated Protein 25)
Catalog No USB-S1014-71C
Supplier’s Catalog No S1014-71C
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 23
Storage -20°C
Other names
Grade Highly Purified
Purity ≥ 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Form Supplied as a liquid in 20mM Tris-HCl pH 7.5, 50mM sodium chloride, 5mMDTT, 1mM EDTA and 10% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity ≥ 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Description SNAP-25 is a presynaptic plasma membrane protein essential for synaptic vesicle fusion and neurotransmitter release . The membrane proteins SNAP-25, synaptobrevin and syntaxin make up the core complex (SNARE) essential for docking and holding synaptic vesicles at the pre synaptic membrane. Description: SNAP-25 gene was amplified by PCR from C.elegans and cloned into an E. coli expression vector. SNAP-25 was purified by using conventional chromatography techniques. Recombinant C.elegans SNAP-25 produced in E.Coli is a single,non-glycosylated polypeptide chain containing 207 amino acids and having a molecular mass of 23kD. Sequence: MSGDDDIPEG LEAINLKMNA TTDDSLESTR RMLALCEESK EAGIKTLVML DDQGEQLERCEGALDTINQD MKEAEDHLKG MEKCCGLCVL PWNKTDDFEK TEFAKAWKKD DDGGVISDQPRITVGDSSMG PQGGYITKIT NDAREDEMDE NVQQVSTMVG NLRNMAIDMS TEVSNQNRQL DRIHDKAQSN EVRVESANKR AKNLITK Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.