Syntaphilin, Recombinant, Human (SNPH, bA314N13.5, KIAA0374, MGC46096)

Catalog No : USB-S9116-08
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Product name Syntaphilin, Recombinant, Human (SNPH, bA314N13.5, KIAA0374, MGC46096)
Catalog No USB-S9116-08
Supplier’s Catalog No S9116-08
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity Human,
Applications
Molecular weight 48.2
Storage -70°C
Other names
Grade Highly Purified
Purity Purified ≥ 90% by SDS-PAGE.
Form Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 1mM DTT, 10 % glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity Purified ≥ 90% by SDS-PAGE.
Description Syntaphilin is a neuron-specific protein initially characterized as a binding partner of syntaxin-1. The protein competes with SNAP-25 for binding to syntaxin-1 and prevents the formation of the SNARE core complex, thereby controlling free syntaxin-1 availability for the assembly of the SNARE complex and potentially regulating synaptic vesicle exocytosis. Recombinant Syntaphilin protein was expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant corresponding to aa1-424 of human Syntaphilin (His-tagged) expressed in E.coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MAMSLPGSRR TSAGSRRRTS PPVSVRDAYG TSSLSSSSNS GSYKGSDSSP TPRRSMKYTL CSDNHGIKPP TPEQYLTPLQ QKEVCIRHLK ARLKDTQDRL QDRDTEIDDL KTQLSRMQED WIEEECHRVE AQLALKEARK EIKQLKQVID TVKNNLIDKD KGLQKYFVDI NIQNKKLETL LHSMEVAQNG MAKEDGTGES AGGSPARSLT RSSTYTKLSD PAVCGDRQPG DPSSGSAEDG ADSGFAAADD TLSRTDALEA SSLLSSGVDC GTEETSLHSS FGLGPRFPAS NTYEKLLCGM EAGVQASCMQ ERAIQTDFVQ YQPDLDTILE KVTQAQVCGT DPESGDRCPE LDAHPSGPRD PNSAVVVTVG DELEAPEPIT RGPTPQRPGA NPNPGQSVSV VCPMEEEEEA AVAEKEPKSY WSRH Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1mg/ml 15% SDS-PAGE , ~48.2kD Optimal dilutions to be determined by the researcher. Storage and Stability: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.