NEUREXIN 1 (CT) (NRXN1, DKFZp313P2036, FLJ35941, Hs.22998, KIAA0578, Neurexin-1-alpha precursor, Neurexin-1-beta precursor, Neurexin I-alpha, Neurexin I-beta) (Control Peptide)

Catalog No : USB-N2110-04A-P
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Product name NEUREXIN 1 (CT) (NRXN1, DKFZp313P2036, FLJ35941, Hs.22998, KIAA0578, Neurexin-1-alpha precursor, Neurexin-1-beta precursor, Neurexin I-alpha, Neurexin I-beta) (Control Peptide)
Catalog No USB-N2110-04A-P
Supplier’s Catalog No N2110-04A-P
Supplier US Biologicals
Source antigen Synthetic
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -20°C
Other names
Grade Highly Purified
Purity Highly Purified
Form Supplied as a lyophilized powder in PBS. Reconstitution: Reconstitute with 200ul sterile ddH2O.
Reactivity life 12 months
Note For reserch purpose only
Purity Highly Purified
Description C-KEKQPSSAKSSNKN from the C-Terminal region of NRXN1. Control peptide corresponding to polyclonal antibody, Catalog#N2110-04A, goat x human. Neurexin 1 (NRXN1) is a transmembrane neuronal protein and member of the neurexin family, which act as cell adhesion molecules and receptors within the vertebrate nervous system. NRXN1 exists in many distinct isoforms, the majority of which encode the longer alpha-neurexins generated using an upstream promoter, whilst fewer encode the shorter beta-neurexins, generated using a downstream promoter. Alpha-neurexins have been shown to bind with high affinity to the neuronal protein neurexophilin, whilst neuroligin 1, enriched in synaptic plasma membranes, has been identified as a splice site-specific ligand for beta-neurexins. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking (see corresponding antibody N2110-04A): Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.