NudE-L (NudE-like Protein, Nudel, Nuclear Distribution Gene E Like Homolog 1, NDEL1, DKFZp451M0318, Endooligopeptidase A, EOPA, Mitosin-associated Protein 1, Mitosin-associated Protein MITAP1) (Control Peptide)
Catalog No : USB-N7400-30D-P
421.85€
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| Product name | NudE-L (NudE-like Protein, Nudel, Nuclear Distribution Gene E Like Homolog 1, NDEL1, DKFZp451M0318, Endooligopeptidase A, EOPA, Mitosin-associated Protein 1, Mitosin-associated Protein MITAP1) (Control Peptide) | ||
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| Catalog No | USB-N7400-30D-P | ||
| Supplier’s Catalog No | N7400-30D-P | ||
| Supplier | US Biologicals | ||
| Source antigen | Synthetic | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | Highly Purified | ||
| Form | Supplied as a lyophilized powder in PBS. Reconstitution: Reconstitute with 200ul sterile ddH2O. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Highly Purified | ||
| Description | CRNFAKDQASRKSY, from the internal region of human NDEL1. Control peptide corresponding to polyclonal antibody, Catalog#N7400-30D, goat x human. Nudel, or endooligopeptidase A, a protein highly expressed in the brain where it associates with dynein motors and Lis1, both of which have been linked to neuronal development and migration. Nudel facilitates the polymerisation of neurofilaments and the organisation of the cellular microtubule array and microtubule anchoring at the centrosome. It is required for several dynein- and microtubule-dependent processes such as the centripetal motion of secretory vesicles, coupling of the nucleus and centrosome and maintaining Golgi integrity. Nudel also serves as a Cdk5 substrate and its phosphorylation status affects its localisation in neurons. Applications: Suitable for use in ELISA. Other Applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher Positive Control: Human Brain (Cerebellum) lysates Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking (see corresponding antibody N7400-30D): Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel. | ||
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