Synaptosomal-associated Protein 25kD, Recombinant, Human, His-Tag (SNAP25)

Catalog No : USB-209221
473.58€
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Product name Synaptosomal-associated Protein 25kD, Recombinant, Human, His-Tag (SNAP25)
Catalog No USB-209221
Supplier’s Catalog No 209221
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 25.4
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (RP-HPLC, SDS-PAGE)
Form Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 2mM EDTA, 50mM sodium chloride, 1mM DTT.
Reactivity life 12 months
Note For reserch purpose only
Purity ~95% (RP-HPLC, SDS-PAGE)
Description Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, SNAP25 product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. The synaptosomal-associated protein (SNAP-25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP-25 is directly involved in the release of neurotransmitters. Source: Recombinant protein corresponding to human SNAP25, a single, non-glycosylated polypeptide chain containing 226aa, fused to His-tag at N-terminal, expressed in E. coli. Molecular Weight: ~25.4kD Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.