Inducible Nitric Oxide Synthase, Control Peptide (iNOS, iNOS 2, mNOS)
Catalog No : USB-I0800-09A
417.25€
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| Product name | Inducible Nitric Oxide Synthase, Control Peptide (iNOS, iNOS 2, mNOS) | ||
|---|---|---|---|
| Catalog No | USB-I0800-09A | ||
| Supplier’s Catalog No | I0800-09A | ||
| Supplier | US Biologicals | ||
| Source antigen | Mouse synthetic peptide | ||
| Reactivity | Synthetic peptide mapping to 14 amino acids within the C-terminal of mouse nitric oxide synthase. No significant sequence homology of iNOS-P is seen with NOS-1/NOS-3 or other proteins. Species homology: Human and rat (85%, 12/14 aa). Human, rat and mouse iNOS/NOS-2 are ~1145aa proteins. | ||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | Purified | ||
| Form | Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Purified | ||
| Description | Control peptide for I0800-09 (antiserum) and I0800-09B (affinity purified antibody). Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues. NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS, I-III, MW ~130-160kD). One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA). It is found in endothelium, adrenal glands, brain and platelets. The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA. It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II). Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible. Sequence homology among different cloned isoforms is ~ 50%. Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested. Recommended Dilution: ELISA: Coat at 1ug/ml. Antibody Blocking: 5-10ug per 1ul I0800-09 (antiserum) or per 1ug I0800-09B (affinity purified antibody). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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