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Categories: Peptides & Proteins / Proteins

Product name			CETN1, Recombinant, Human, aa1-172, His-Tag (Centrin-1, Caltractin Isoform 2, CEN1, CETN)
Catalog No			USB-045414
Supplier’s Catalog No			045414
Supplier			US Biologicals
Source antigen			Recombinant, E.coli
Reactivity
Cross reactivity
Applications
Molecular weight			22.1

Storage			-20°C
Other names
Grade			Highly Purified
Purity			~90% (SDS-PAGE)
Form			Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.1M sodium chloride, 10% glycerol.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~90% (SDS-PAGE)
Description			Centrin, also known as CETN1, is an EF-hand type Ca2+-binding protein. It is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle. CETN1 plays an important role in the determination of centrosome position and segregation, and in the process of microtubule severing. Source: Recombinant corresponding to aa1-172 from human CETN1, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~22.1kD (196aa) confirmed by MALDI-TOF AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMASGFK KPSAASTGQK RKVAPKPELT EDQKQEVREA FDLFDVDGSG TIDAKELKVA MRALGFEPRK EEMKKMISEV DREGTGKISF NDFLAVMTQK MSEKDTKEEI LKAFRLFDDD ETGKISFKNL KRVANELGEN LTDEELQEMI DEADRDGDGE VNEEEFLRIM KKTSLY Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.