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Categories: Peptides & Proteins / Proteins

Product name			MINA, Recombinant, Human, aa1-465, His-Tag (MYC Induced Nuclear Antigen)
Catalog No			USB-218341
Supplier’s Catalog No			218341
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			54.9

Storage			-20°C
Other names
Grade			Purified
Purity			~90% (SDS-PAGE)
Form			Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15M sodium chloride, 1mM DTT, 10% glycerol.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~90% (SDS-PAGE)
Description			MINA is an oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. This protein is involved in the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. It also catalyzes the hydroxylation of 60S ribosomal protein L27a on 'His-39'. MINA may play an important role in cell growth and survival. It may be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles. Source: Recombinant protein corresponding to aa1-465 from human MINA, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~54.9kD (485aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.