DNA, ds (Plasmid)
Catalog No : USB-D4015-06
991.98€
0.00€
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| Product name | DNA, ds (Plasmid) | ||
|---|---|---|---|
| Catalog No | USB-D4015-06 | ||
| Supplier’s Catalog No | D4015-06 | ||
| Supplier | US Biologicals | ||
| Source antigen | |||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -70°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | Purified by isolating the Plasma DNA from E. coli by alkaline lysis and chromatographic methodologies. A260nm: 1.0 at 50ug/ml | ||
| Form | Supplied as a liquid in 0.01M Tris, 0.001M EDTA, pH 8.0. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Purified by isolating the Plasma DNA from E. coli by alkaline lysis and chromatographic methodologies. A260nm: 1.0 at 50ug/ml | ||
| Description | The dsDNA antigen is a 2690bp plasmid purified by alkaline lysis and chromatography. The purification process includes operations to minimize reactivity to antibodies against single-stranded DNA. Use of plasmid DNA in ELISA is an effective method for the detection of anti-double-stranded (ds) DNA antibodies (1). The presence of antibodies to dsDNA is included in the 1982 revised criteria for classification of Systemic Lupus Erythematosus (SLE) (2). Approximately 60-70% of SLE patients have antibodies to dsDNA, and there is considerable evidence to implicate immune complexes containing anti-dsDNA and DNA in the pathogenesis of SLE (3,4). In addition, levels of anti-dsDNA antibodies have been demonstrated to correlate with disease activity in many patients (5,6). Low levels of anti-dsDNA antibodies may occur in other rheumatic diseases (7) and may occur at a very low frequency (<2%) in individuals without any symptoms of rheumatic disease (8). Anti-dsDNA antibodies may appear in rheumatic patients prior to the development of disease manifestations (9). Applications: Suitable for use in standard solid phase immunoassays. Other applications not tested. Purity: Purified by isolating the Plasma DNA from E. coli by alkaline lysis and chromatographic methodologies. Concentration: ~1mg/ml Form: Supplied as a liquid in 0.01M Tris, 0.001M EDTA, pH 8.0. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months. | ||
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