Single-Stranded DNA Binding Protein, E. coli (SSB)
Catalog No : USB-S1013-86R
304.61€
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| Product name | Single-Stranded DNA Binding Protein, E. coli (SSB) | ||
|---|---|---|---|
| Catalog No | USB-S1013-86R | ||
| Supplier’s Catalog No | S1013-86R | ||
| Supplier | US Biologicals | ||
| Source antigen | E. coli strain containing an overproducing clone of E. coli SSB protein. | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -70°C | ||
|---|---|---|---|
| Other names | |||
| Grade | |||
| Purity | ≥95% (SDS-PAGE) | ||
| Form | Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 200mM sodium chloride, 0.1mM EDTA, 1mM DTT, 50% glycerol | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥95% (SDS-PAGE) | ||
| Description | E. coli Single-Stranded DNA Binding Protein (SSB) consists of four identical 18.9kD subunits. It binds with high affinity in a cooperative manner to single-stranded DNA but does not bind well to double-stranded DNA. It is involved in DNA replication and in recombination in vivo. Spectral Ratio A280/A260: As Reported Binding Activity: ~5ug of SSB protein is required to prevent optical density change of 1ug of single-stranded DNA upon addition of 10mM MgCl2. Isoelectric Point: 6.0 Nucleotides Bound Per Monomer: 8-16 Thermostability: SSB retains activity after 20 min incubation at 65°C. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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