Leptin Receptor B, Mouse, phosphorylated, (Tyr985) (ObRb, Obese Receptor B) (Control Peptide)
Catalog No : USB-L1671-12A
417.25€
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| Product name | Leptin Receptor B, Mouse, phosphorylated, (Tyr985) (ObRb, Obese Receptor B) (Control Peptide) | ||
|---|---|---|---|
| Catalog No | USB-L1671-12A | ||
| Supplier’s Catalog No | L1671-12A | ||
| Supplier | US Biologicals | ||
| Source antigen | Mouse synthetic peptide | ||
| Reactivity | Specific for the long isoform (OBRb). 100% conserved in rat, 91% in human and monkey OBRb. | ||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | Highly purified | ||
| Form | Supplied as a liquid in PBS, pH 7.2 | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Highly purified | ||
| Description | Obesity, a common nutritional disorder, is associated with diabetes, hypertension, hyperlipidemia, cancer and many other health related problems. Obese gene, leptin, mediates its effects via the Leptin receptor or obese receptors (OBR or LR) that is expressed in several tissues including hypothalamus. The OBR has at least 6 alternatively spliced forms (OBRa-f or LRa-f) that contain a common extracellular domain. OBRb or long isoform contains a unique ~ 300 aa long cytoplasmic tail that is required for leptin signaling. OBR is abnormally spliced (truncation of cytoplasmic domain) in db/db mice resulting into severe obesity. Leptin is structurally related to cytokine family and it activates cytokine-like signal transduction by stimulating the JAK-STAT pathway via the OBRb, a member of IL-6 receptor family of class I cytokine receptors. Leptin binding to OBRb results in the activation of JAK2 and subsequent phosphorylation of specific tyrosines (Tyr985 and Tyr1138) located at the C-terminal, cytoplasmic region of OBRb. Phosphorylation of OBRb Tyr1138 recruits the SH2 domain containing STAT3, its autophosphorylation, translocation to the nucleus, and modulation of gene transcription, most notably induction of suppressor of cytokine signaling 3 gene (SOCS3 or CIS3). SOCS3 inhibits leptin signaling. SOCS3 have been reported to be elevated in some obese animals. Tyr985 phosphorylation of OBRb recruits the SH2 domain containing protein-tyrosine phosphatase SHP-2, it's binding to GRB2, and subsequent activation of ERK, a group of ser/thr kinases involved in the regulation of gene transcription. Therefore, OBRb Tyr phosphorylation plays in critical role in leptin signaling. Applications: Suitable for use in ELISA and Antibody Blocking. Other applications not tested. Recommended Dilutions: ELISA: 10-100 ng per well using 0.1-2ug/ml antibody Antibody Blocking: 5-10ug per 1ug of affinity purified IgG (L1671-12). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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