Leptin Receptor B, Mouse, phosphorylated, (Tyr985) (ObRb, Obese Receptor B) (Control Peptide)
Catalog No : USB-L1671-12C
417.25€
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| Product name | Leptin Receptor B, Mouse, phosphorylated, (Tyr985) (ObRb, Obese Receptor B) (Control Peptide) | ||
|---|---|---|---|
| Catalog No | USB-L1671-12C | ||
| Supplier’s Catalog No | L1671-12C | ||
| Supplier | US Biologicals | ||
| Source antigen | Mouse synthetic peptide | ||
| Reactivity | 100% conserved in rat, 91% in human and monkey OBRb. Can be used as suitable controls to demonstrate antibody specificity to confirm antibody specificity in various assay. Control peptides, because of their small size (2-3kD), is not recommended for Western. The peptides can also be used in ELISA demonstrate antibody specificity | ||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
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| Grade | Highly Purified | ||
| Purity | Highly purified | ||
| Form | Supplied as a liquid in PBS, pH 7.2 | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Highly purified | ||
| Description | Obesity, a common nutritional disorder, is associated with diabetes, hypertension, hyperlipidemia, cancer and many other health related problems. Obese gene, leptin, mediates its effects via the Leptin receptor or obese receptors (OBR or LR) that is expressed in several tissues including hypothalamus. The OBR has at least 6 alternatively spliced forms (OBRa-f or LRa-f) that contain a common extracellular domain. OBRb or long isoform contains a unique ~ 300 aa long cytoplasmic tail that is required for leptin signaling. OBR is abnormally spliced (truncation of cytoplasmic domain) in db/db mice resulting into severe obesity. Leptin is structurally related to cytokine family and it activates cytokine-like signal transduction by stimulating the JAK-STAT pathway via the OBRb, a member of IL-6 receptor family of class I cytokine receptors. Leptin binding to OBRb results in the activation of JAK2 and subsequent phosphorylation of specific tyrosines (Tyr985 and Tyr1138) located at the C-terminal, cytoplasmic region of OBRb. Phosphorylation of OBRb Tyr1138 recruits the SH2 domain containing STAT3, its autophosphorylation, translocation to the nucleus, and modulation of gene transcription, most notably induction of suppressor of cytokine signaling 3 gene (SOCS3 or CIS3). SOCS3 inhibits leptin signaling. SOCS3 have been reported to be elevated in some obese animals. Tyr985 phosphorylation of OBRb recruits the SH2 domain containing protein-tyrosine phosphatase SHP-2, it's binding to GRB2, and subsequent activation of ERK, a group of ser/thr kinases involved in the regulation of gene transcription. Therefore, OBRb Tyr phosphorylation plays in critical role in leptin signaling. | ||
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