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Categories: Peptides & Proteins / Proteins

Product name			Leptin, Recombinant, Mouse (LEP, Ob, Obesity Factor)
Catalog No			USB-L1670-10D
Supplier’s Catalog No			L1670-10D
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			16

Storage			-20°C
Other names
Grade			Highly Purified
Purity			~95% (SDS-PAGE). Endotoxin: ≤0.1ng/ug.
Form			Supplied as a liqud in 10mM sodium chloride, pH 5.2.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~95% (SDS-PAGE). Endotoxin: ≤0.1ng/ug.
Description			Leptin (Anti-Obesity Protein) is the protein product of the ob (obese) gene in mice and seems to be involved in appetite control. The ob gene is expressed in adipose tissue and is thought to regulate the body’s fat stores. Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non-insulin dependent) diabetes. Murine Leptin is a 16kD protein containing 147 amino acid residues. Source: Recombinant mouse Leptin expressed in E. coli. Biological Actvity: Murine Leptin has been shown to be biologically active in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5ug Leptin/gm of body weight for 14 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.