Leptin, Recombinant, Human (LEP, Leptin Mouse Obesity Homolog, Leptin Precursor Obesity Factor, FLJ94114, Ob, Obese Protein, Obesity Factor, Obesity Homolog Mouse, OBS)
Catalog No : USB-L1670-17G
471.28€
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| Product name | Leptin, Recombinant, Human (LEP, Leptin Mouse Obesity Homolog, Leptin Precursor Obesity Factor, FLJ94114, Ob, Obese Protein, Obesity Factor, Obesity Homolog Mouse, OBS) | ||
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| Catalog No | USB-L1670-17G | ||
| Supplier’s Catalog No | L1670-17G | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 16 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~98% (SDS-PAGE and HPLC). Endotoxin: ≤ 0.1ng/ug. | ||
| Form | Supplied as a lyophilized powder. Reconstitute with sterile 15mM HCl to ~2mg/ml. After completely dissolved, add 7.5mM sterile NaOH (300ul/1mg) and bring the pH to ~5.2. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~98% (SDS-PAGE and HPLC). Endotoxin: ≤ 0.1ng/ug. | ||
| Description | Leptin (Anti-Obesity Protein) is the protein product of the ob (obese) gene in mice and seems to be involved in appetite control. The ob gene is expressed in adipose tissue and is thought to regulate the body’s fat stores. Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non-insulin dependent) diabetes. Human Leptin is a 16kD protein containing 146 amino acid residues. Source: Recombinant human Leptin expressed in E. coli. Biological Activity: Biologically active in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5ug Leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline- treated controls. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile HCl. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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