AgRP, Recombinant, Human, His-Tag (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related Transcript)
Catalog No : USB-208725
531.05€
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| Product name | AgRP, Recombinant, Human, His-Tag (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related Transcript) | ||
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| Catalog No | USB-208725 | ||
| Supplier’s Catalog No | 208725 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 14.4 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (SDS-PAGE). Three-step procedure using IMAC and size exclusion chromatography before and after refolding. | ||
| Form | Supplied as a lyophilized powder in 5mM Tris, pH 7.5, 25mM sodium chloride. No preservative added. Reconstitute with 200ul sterile ddH2O and let the lyophilized pellet dissolve completely. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (SDS-PAGE). Three-step procedure using IMAC and size exclusion chromatography before and after refolding. | ||
| Description | Agouti-related protein is an endogenous antagonist of hypothalamic alpha-melanocortin receptors MC3R and MC4R with potent orexigenic activity. Although a complete deletion of the AGRP gene does not produce any significant metabolic phenotypes, reduction in AGRP expression by RNA interference is associated with increased metabolic rate along with reduced weight gain. In hypothalamus, it is produced by neurons in the medial portion of arcuate nucleus, which produce also the potent orexigenic peptide Neuropeptide Y (NP-Y). Another site of central AGRP production is the hypothalamic nucleus. AGRP encompasses 132aa residues and its alpha-melanocortin inhibiting activity results in a 34aa cystine knot domain within the C-terminal (87-132) portion of the protein. Both AGRP and NP-Y expression was shown to be suppressed by leptin. Central administration of AGRP induces hyperphagia and increased gain in body weight in rodents, but may also exert metabolic effects even when hyperphagia is prevented. In the absence of hyperphagia, intracerebralventricular administration of AGRP caused significant increases in plasma leptin and insulin concentrations (two fold and 1.5-fold, respectively) and fat pad mass. In the periphery, AGRP mRNA was found in adrenal glands, lung, testis, ovary, skeletal muscle and adipose tissue in humans or rodents. In the adrenals, it was shown that AGRP antagonizes glucosteroid production mediated by MC4R. AGRP could then modulate locally the functions of some peripheral tissues such as adrenals. In human and rat serum, detectable levels of AGRP-like activity were reported in the lower picogram range. The serum AGRP levels were elevated in obese humans compared to lean controls and increased with fasting in rats. Source: Recombinant protein corresponding to human AgRP, containing 112aa residues, fused to his-tag at N-terminal, expressed in E. coli. Molecular Weight: ~14.4kD Specificity: The aa sequence of the recombinant human Agouti–related protein is 100% homologous to the aa sequence of the human Agouti–related protein without signal sequence. AA Sequence: MKHHHHHHHM LVPRGSAQMG LAPMEGIRRP DQALLPELPG LGLRAPLKKT TAEQAEEDLL QEAQALAEVL DLQDREPRSS RRCVRLHESC LGQQVPCCDP CATCYCRFFN AFCYCRKLGT AMNPCSRT Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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