Osteonectin, Recombinant, Human (SPARC, Secreted Protein Acidic and Rich in Cysteine, BM-40, ON)
Catalog No : USB-220118
381.62€
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| Product name | Osteonectin, Recombinant, Human (SPARC, Secreted Protein Acidic and Rich in Cysteine, BM-40, ON) | ||
|---|---|---|---|
| Catalog No | USB-220118 | ||
| Supplier’s Catalog No | 220118 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, CHO cells | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 43.7 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~97% (SDS-PAGE) | ||
| Form | Supplied as a lyophilized powder in 10mM sodium phosphate, pH 7.6. No preservative added. Reconstitute with sterile ddH2O to 0.1-1mg/ml. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~97% (SDS-PAGE) | ||
| Description | SPARC/Osteonectin is a secreted, evolutionarily conserved collagen-binding glycoprotein that is involved in a variety of cellular activities. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC/Osteonectin and its related peptides bind to numerous proteins of the extracellular matrix (ECM), affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC/Osteonectin consists of three domains; an N-terminal acidic region that binds calcium ions with low affinity, a module containing two EF-hand motifs that bind calcium with high affinity, and a cysteine-rich follistatin-like domain. Source: Recombinant protein corresponding to 286aa from human Osteonectin, expressed in CHO cells. Molecular Weight: ~43.7kD, by SDS-PAGE analysis due to the effect of glycosylation Biological Activity: Determined by its ability to increase alkaline phosphatase activity in differentiating MC3T3 cells using a concentration of 0.5-0.7ug/ml. Endotoxin: <0.1ng/ug of protein (<1EU/ug) AA Sequence: APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVI Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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