Agarase, beta, Recombinant (b-Agarase)
Catalog No : USB-219344
474.73€
0.00€
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| Product name | Agarase, beta, Recombinant (b-Agarase) | ||
|---|---|---|---|
| Catalog No | USB-219344 | ||
| Supplier’s Catalog No | 219344 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 36 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | |||
| Purity | |||
| Form | |||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Description | The agarase is a recombinant protein from P. atlantica produced in E. coli. Agarase cleaves agarose to neoagaro-oligosaccharides, and is used for quantitative, gentle recovery of DNA from low melting point agarose. Molecular Weight: ~36kD Concentration: 2units/ul Components: 219344: Agarase, beta, Recombinant, 1x500u Supplied as a liquid in 40mM Tris-HCl, pH 7.5, 50mM sodium chloride, 50% glycerol. 219344A: Buffer, 10X, 1x2ml Supplied as a liquid 1M Tris-HCl pH 6.5, 10 mM EDTA Unit Definition: One unit is defined as the amount of enzyme required to digest 100ul (~100mg) of molten 1% low melting point agarose to neoagaro-oligosaccharides in 1 hour at 45°C. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. RECOVERY OF DNA FROM LOW MELTING POINT AGAROSE GEL: 1. Cut DNA band of interest from the low melting point agarose gel under a long-wave UV light, and transfer the agarose piece into a tared eppendorf tube. 2. Determine the weight of the agarose piece and add 0.1 volumes of 219344B: Buffer, 10X. Incubate the tube for 15 min at 65°C until the agarose is completely molten. A clear, nonviscous solution is required to be sure that all cleavage sites are accessible to the agarase. 3. Cool down the melten agarose to 42-45°C and add 1 unit 219344A: Agarase, beta, Recombinant per 100mg of agarose (100ul 1% agarose in Tris-acetate). Note: When the percentage of agarose in higher than 1%, the units of agarase have to be adjusted in proportion. When using Tris-borate gel electrophoresis buffer, a two-fold amount of agarase should be used; otherwise,the incubation time has to be prolonged. 4. Carefully mix the solution and incubate for 1 hour at 42-45°C. 5. Add 0.1 volume of 3M sodium acetate, pH 5.5, to the melted agarose solution and incubate 15 min on ice 6. Centrifuge for 15 minutes at 4°C to pellet the oligosacchrides. 7. Precipitate the nucleic acids from the supernatant with 3 volume of ice-cold ethanol as usual. | ||
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