Pregnane X Receptor (PXR, BXR, Nuclear Receptor Subfamily 1 Group I Member 2, NR1I2, ONR1, PAR1, PAR2, PAR, PARq, PRR, SAR, Steroid and Xenobiotic Receptor, SXR) (Control Peptide)
Catalog No : USB-P6001-30A-P
421.85€
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| Product name | Pregnane X Receptor (PXR, BXR, Nuclear Receptor Subfamily 1 Group I Member 2, NR1I2, ONR1, PAR1, PAR2, PAR, PARq, PRR, SAR, Steroid and Xenobiotic Receptor, SXR) (Control Peptide) | ||
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| Catalog No | USB-P6001-30A-P | ||
| Supplier’s Catalog No | P6001-30A-P | ||
| Supplier | US Biologicals | ||
| Source antigen | Synthetic | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | Highly Purified | ||
| Form | Supplied as a lyophilized powder in PBS. Reconstitution: Reconstitute with 200ul sterile ddH2O. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Highly Purified | ||
| Description | EQFAITLKSYIECNR, from the internal region of human PXR. Control peptide corresponding to polyclonal antibody, Catalog#P6001-30A, goat x human. This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Diltuions: Optimal dilutions to be determined by the researcher Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking (see corresponding antibody P6001-30A): Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel. | ||
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