Imugex

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Categories: Peptides & Proteins / Proteins

Product name			Actin, beta (Non-muscle), Control Peptide
Catalog No			USB-A0760-40K1
Supplier’s Catalog No			A0760-40K1
Supplier			US Biologicals
Source antigen			Synthetic peptide
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			-20°C
Other names
Grade			Purified
Purity			Purified
Form			Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Reactivity life			12 months
Note			For reserch purpose only
Purity			Purified
Description			Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (g- smooth muscle and g-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots. Source: Synthetic peptide corresponding to N-terminal 15aa of human beta-actin, non-muscle. Applications: Suitable for use in Antibody Blocking and ELISA. Other applications not tested. Recommended Dilution: ELISA: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.