Pap Pen, Mini

Catalog No : USB-P3102-50
400.01€
0.00€

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Product name Pap Pen, Mini
Catalog No USB-P3102-50
Supplier’s Catalog No P3102-50
Supplier US Biologicals
Source antigen
Reactivity
Cross reactivity
Applications
Molecular weight
Storage RT
Other names
Grade
Purity
Form
Reactivity life 12 months
Note For reserch purpose only
Description The Mini PAP pen draws a water repellent circle around slide mounted tissue, preventing the waste of valuable reagents by keeping the liquid pooled in a single droplet. The Mini PAP Pen is especially useful when working with multiple sections on a single slide because its tip is smaller than the PAP pen. Each pen can be used to draw about 400 circles. Applications: Suitable for use in Immunohistochemistry and fluorescent staining methods. Other applications not tested. Recommended Usage: 1) For Paraffin-embedded sections: - Deparaffinize and rehydrate tissue sections. - Rinse tissue sections with PBS and wipe off excess liquid around the tissue. - Draw a circle on the glass slide around the section with Mini PAP pen. - Dry the circle for 1-2 minutes at RT. - Continue the normal immunohistostaining process. 2) For Frozen sections: - Preclean glass slide. - Coat slide with H5107: HistoGrip (50X concentrate) or other adhesive media (Optional). - Cut and mount tissue sections on the slides. - Rinse tissue sections with PBS and wipe off excess liquid around the tissue. - Draw a circle on the glass slide around the section with Mini PAP pen. - Dry the circle for 1-2 minutes at RT. - Continue the normal immunohistostaining process. Note: The water repellent circle is removable in xylene or xylene substitutes. Since it is not influenced by water soluble media, it will not interfere with microscopic study. Storage and Stability: Store at RT with cap on tight and tip pointed down. Press down on tip when pen starts to dry out.