Imugex

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Categories: Biochemicals / Biochemicals

Product name			ImmunoTek™ TMB (3,3',5,5'-Tetramethylbenzidine) Stable Liquid Substrate for HRPO, 1.25mM, Soluble
Catalog No			USB-T6005
Supplier’s Catalog No			T6005
Supplier			US Biologicals
Source antigen			Synthetic
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			4°C
Other names
Grade			Molecular Biology Grade
Purity
Form
Reactivity life			12 months
Note			For reserch purpose only
Description			ImmunoTek™ TMB (3,3',5,5'-Tetramethylbenzidine) Stable Liquid Substrate for HRPO, 1.25mM, Soluble Tetramethylbenzidine (TMB) is an excellent substrate for detecting horseradish peroxidase (HRP) labeled probes. TMB produces a soluble end product which is blue in color that is quantitated at 650nm, providing an ultrasensitive quantitative substrate system. TMB has been shown to be a safe-sensitive substrate for the assay of horseradish peroxidase (HRP). Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color wth an adsorption maximum at 653nm. Further reaction with HRP/H2O2 or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing light at 450 nm. The extinction coefficient of the radical (E653 nm = 5.9x10e4 mol-1 cm-1) provide a remarkably sensitive system for the assay of HRP and HRP labeled probes. TMB is a single component reagent stable at room temperature and not sensitive to normal laboratory light. It is optimized with respect to TMB and hydrogen peroxide concentrations and yields a linear response with the concentrations of HRP usually employed in immunologic assays.