Imugex

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Categories: Biochemicals / Biochemicals

Product name			ImmunoTek™ ABTS Stable Liquid Substrate 1.46mM for HRPO
Catalog No			USB-A0030
Supplier’s Catalog No			A0030
Supplier			US Biologicals
Source antigen			Horseradish peroxidase
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			RT/4°C
Other names
Grade			Molecular Biology Grade
Purity
Form
Reactivity life			12 months
Note			For reserch purpose only
Description			ImmunoTek™ ABTS Stable Liquid Substrate 1.46mM for HRPO ABTS is a soluble, safe, convenient, and good overall performing substrate for horseradish peroxidase (HRP) based ELISA assays. ABTS develops an intense blue-green color measurable between 405nm and 410nm. It may be used for both kinetic and endpoint reactions. ABTS (1.46mM) is supplied in citrate buffer, pH 4.0. In the presence of hydrogen peroxide and HRP, ABTS is oxidized to a radical cation showing absorption maxima at 820nm, 734nm, 650nm, and 405nm. The latter frequency demonstrates a significant molar extinction coefficient and is generally employed for most ABTS assays. Stopping the reaction with acid does not alter the 405nm spectrum allowing kinetic and endpoint methods to be measured at the same wavelength. Using a proprietary, nontoxic stabilization technology, United States Biological provides a room temperature stable, single component ABTS solution satisfactory for quantitative analysis of HRP based systems. Features: • Single component system • Room temperature stable • Exhibits low background levels • Safe to use-no weighing of hazardous powders • Save preparation time-use right out of the bottle • Ultra-low sensitivity • Minimal lot-to-lot variation