p300, Western Blot Positive Control
Catalog No : USB-P1005-10A
531.05€
0.00€
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| Product name | p300, Western Blot Positive Control | ||
|---|---|---|---|
| Catalog No | USB-P1005-10A | ||
| Supplier’s Catalog No | P1005-10A | ||
| Supplier | US Biologicals | ||
| Source antigen | GST fusion protein | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | Highly Purified (~95%) | ||
| Form | Supplied as a lyophilized powder from PBS, 0.05% sodium azide. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Highly Purified (~95%) | ||
| Description | Control peptide for P1005-10. The transcriptional coactivator and integrator p300 and its closely related family member CREBBP mediate multiple signal-dependent transcriptional events. CREB binding protein (CBP) and p300 were both identified initially in protein interaction assays-the former through its association with the transcription factor CREB and the latter through its interaction with the adenoviral-transforming protein E1A. p300 contains 3 cysteine- and histidine-rich regions of which the most carboxy-terminal region interacts specifically with E1A. In its center, p300 contains a bromodomain, a hallmark of certain transcriptional coactivators. p300 and CREB-binding protein are highly related in primary structure. Several protein motifs such as a bromodomain, a KIX domain, and 3 regions rich in cys/his residues are well conserved between these 2 proteins. P300 protein is also a histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. Animals nullizygous for p300 died between days 9 and 11.5 of gestation, exhibiting defects in neurulation, cell proliferation, and heart development. Cells derived from p300-deficient embryos displayed specific transcriptional defects and proliferated poorly. These results provide evidence that a coactivator endowed with histone acetyltransferase activity is essential for mammalian cell proliferation and development. p300 is also a targeted by viral oncoproteins. it may also have a role in DNA repair synthesis through its interaction with proliferating cell nuclear antigen (PCNA). Source: A GST fusion protein containing the C-terminus of human p300. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 10 ul/lane. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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