TWIST2, Control Peptide (Twist-related Protein 2, Class A Basic Helix-loop-helix Protein 39, bHLHa39, Dermis-expressed Protein 1, Dermo-1, BHLHA39, DERMO1)

Catalog No : USB-T9300-08B
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Product name TWIST2, Control Peptide (Twist-related Protein 2, Class A Basic Helix-loop-helix Protein 39, bHLHa39, Dermis-expressed Protein 1, Dermo-1, BHLHA39, DERMO1)
Catalog No USB-T9300-08B
Supplier’s Catalog No T9300-08B
Supplier US Biologicals
Source antigen Synthetic peptide
Reactivity
Cross reactivity
Applications
Molecular weight 3
Storage -20°C
Other names
Grade Purified
Purity Purified
Form Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Reactivity life 12 months
Note For reserch purpose only
Purity Purified
Description Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by Twist gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Twist. It is thought that during osteoblast development this protein may inhibit osteoblast maturation and maintain cells in a preosteoblast phenotype. Twist and Dermo1, also called Twist1 and Twist2 respectively, were induced by a cytokine signaling pathway that required the dorsal-related protein Rela, a member of the nuclear factor kappa-B family of transcription factors, in mice. Twist1 and Twist2 repressed cytokine gene expression through interaction with Rela. TWIST proteins regulate cytokine signaling by establishing a negative feedback loop that represses the NFKB-dependent cytokine pathway. Source: Synthetic peptide corresponding to 20aa of mouse Twist2, N-terminal. Molecular Weight: ~3kD Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested. Recommended Dilution: ELISA: 5-10ug control peptide per 1ug of aff pure IgG or 1ul antiserum) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.