Imugex

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Categories: Biochemicals / Biochemicals

Product name			ImmunoTek Plus™ Yellow Stop Reagent, Solid (450nm)
Catalog No			USB-172017
Supplier’s Catalog No			172017
Supplier			US Biologicals
Source antigen
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			4°C
Other names
Grade
Purity
Form
Reactivity life			12 months
Note			For reserch purpose only
Description			ImmunoTek Plus™ Yellow Stop Reagent, Solid (450nm) This is a proprietary dry blend mixture used to stop the one-component TMB and other TMB formulation (3,3’,5,5’-tetramethylbenzidine) reactions by changing the chromogen into the second oxidation state of TMB and form a yellow reaction product read ~450nm. Product Use: This product is used to terminate TMB Microwell substrates and change the absorbance from blue (650nm) to yellow (450nm). Add specified volume (100ml to 1L) of purified water and allow ~5-10 minutes to dissolve. Mix occasionally. Equal volumes of substrate and terminator solution should be used. When TMB peroxidase substrate solution (3,3’,5,5’-tetramethylbenzidine) is reacted with peroxidase, a reaction ensues producing a blue colored liquid product (oxidized state). The reaction can be terminated using proprietary Yellow Stop specifically designed for TMB substrates. Following termination of the reaction, a second oxidized state is produced resulting in a soluble yellow reaction product. The colored product is stable for at least two hours. Storage and Stability: Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.