Interleukin 1 Receptor Antagonist (IL-1Ra)
Catalog No : USB-I7663-25L
377.02€
0.00€
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| Product name | Interleukin 1 Receptor Antagonist (IL-1Ra) | ||
|---|---|---|---|
| Catalog No | USB-I7663-25L | ||
| Supplier’s Catalog No | I7663-25L | ||
| Supplier | US Biologicals | ||
| Source antigen | E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 17 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~98% by RP-HPLC and SDS-PAGE | ||
| Form | Supplied as a sterile filtered, lyophilized powder. No additives. Reconstitute in sterile ddH2O, 0.1% HSA or BSA to >0.1mg/ml. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~98% by RP-HPLC and SDS-PAGE | ||
| Description | Interleukin-1 Receptor Antagonist is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning ~400kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. Recombinant Human IL-1RA produced in E. coli is a non-glycosylated, N-terminal methionyl form of the human naturally-occurring polypeptide chain containing 153 amino acids and having a molecular mass of 17kD. Source: Escherichia coli Purity: ~98% by RP-HPLC and SDS-PAGE Form: Supplied as a sterile filtered, lyophilized powder. No additives. Reconstitute in sterile ddH2O, 0.1% HSA or BSA to >0.1mg/ml. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Arg-Pro-Ser-Gly. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant inhibition of IL-1 stimulation of D10S cells was found to be 0.5ng/ml, corresponding to a specifIc activity of 2,000,000IU/mg. Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 0.89 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics) 2. Analysis by RP-HPLC, using a calibrated solution of IL-8 as a reference standard. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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