Glypican 6, Recombinant, Human (Glypiated Proteoglycan 6)
Catalog No : USB-G8235-58H
705.77€
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| Product name | Glypican 6, Recombinant, Human (Glypiated Proteoglycan 6) | ||
|---|---|---|---|
| Catalog No | USB-G8235-58H | ||
| Supplier’s Catalog No | G8235-58H | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant Human | ||
| Reactivity | |||
| Cross reactivity | Human, | ||
| Applications | |||
| Molecular weight | 3820 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥ 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin ≤ 1EU. | ||
| Form | Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to ≥ 0.1mg/ml. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥ 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin ≤ 1EU. | ||
| Description | The Glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked heparan sulfate (HS) proteoglycans that likely play a key role in embryonic morphogenesis (1, 2, 3, 4). There are currently six known mammalian Glypicans. They all share a common-sized protein core of 60-70kD, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of Glypicans are known, with one subfamily containing Glypicans 1, 2, 4 and 6, and another subfamily containing Glypicans 3 and 5 (3, 5). Human Glypican 6 (GPC-6) is synthesized as a 554 amino acid (aa) preproprecursor that contains a 23 aa signal sequence, a 505 aa mature region and a 26 aa C-terminal prosegment (5, 6). There are four consecutive Ser-Gly repeats that serve as a heparin sulfate attachment site. GPC-6 is reported to be as large as 110kD in size. This translates into approximately 50kD of proteoglycan (5). Human to mouse, there is 97% aa identity over the entire GPC-6 molecule. Cells known to express GPC-6 are adult ovary and embryonic vascular and visceral smooth muscle, plus mesenchyme (embryonic connective tissue) in multiple organs (1, 5, 6). The function of GPC-6 is essentially unknown. As a Glypican family member, it may facilitate heparin-binding growth factor signaling and polyamine uptake into expressing cells (7, 8). In this regard, it would appear that GPC-6 with its attendant HS is downregulated by triiodothyronine during cartilage maturation, thus limiting the availability of sites for FGF sequestration and activity (9). Source: DNA sequence encoding the mature human Glypican 6 (Asp 24-Val 527; Accession # Q9Y625) was fused with six Histidines at the C-terminus and the protein was expressed in a mouse myeloma cell line, NS0. Definition: Measured by its ability to bind biotinylated rmWnt-3A captured on streptavidin-coated plate. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 38kD and 20kD, respectively migrates at ~ 38 and 27-29kD under reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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