FCRN, Recombinant, Mouse, 6-His-Tag (IgG receptor FcRn Large Subunit p51, FcRn, IgG Fc Fragment Receptor Transporter alpha Chain, Neonatal Fc Receptor, Fcgrt)
Catalog No : USB-F0025-83
705.77€
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| Product name | FCRN, Recombinant, Mouse, 6-His-Tag (IgG receptor FcRn Large Subunit p51, FcRn, IgG Fc Fragment Receptor Transporter alpha Chain, Neonatal Fc Receptor, Fcgrt) | ||
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| Catalog No | USB-F0025-83 | ||
| Supplier’s Catalog No | F0025-83 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, NSO cells | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 32.3 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (SDS-PAGE) under reducing conditions, visualized by silver stain Endotoxin: ≤1.0EU/ug of protein (LAL) | ||
| Form | Supplied as a lyophilized powder from PBS, 0.02% Triton, pH 7.5. Reconstitute with 200ul sterile PBS. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (SDS-PAGE) under reducing conditions, visualized by silver stain Endotoxin: ≤1.0EU/ug of protein (LAL) | ||
| Description | The neonatal Fc receptor (FCRN) is an approximately 45kD transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis. Mature mouse FCRN consists of a 276aa extracellular domain (ECD) with two N-terminal alpha domains, one a3/immunoglobulin-like domain, a 24aa transmembrane segment, and a 44aa cytoplasmic domain. Within the ECD, mouse FCRN shares 69% and 91% aa sequence identity with human and rat FCRN, respectively. Mouse FCRN binds with high affinity to IgG from mouse, human, rat, rabbit, guinea pig, bovine, and sheep, while human FCRN binds IgG with significantly lower affinity and is much more restricted in terms of species recognition. It does not bind the structurally related chicken IgG. FCRN additionally binds to albumin, and both it and IgG are bound at pH 5.0 but not at pH 8.0. FCRN associates noncovalently with beta 2-Microglobulin, and this interaction is important for the intracellular trafficking of FCRN. FCRN cycles between the plasma membrane and acidified intracellular compartments of endothelial cells and epithelial cells. It binds endocytosed IgG and albumin in the low pH vesicles and transports them to the plasma membrane for extracellular release at higher pH. This protects IgG and albumin from lysosomal degradation and helps maintain the circulating levels of both proteins. This mechanism is involved in the bidirectional transport of IgG across epithelial and endothelial barriers including neonatal IgG absorption in the intestine and fetal uptake of maternal antibodies through the placenta. In the kidney, FCRN recycles albumin to the serum but removes IgG from the glomular basement membrane and promotes its excretion into the urine. FCRN is also expressed in neutrophils and myeloid antigen presenting cells. It can enhance IgG-mediated phagocytosis and antigen presentation by these cells, but it promotes the degradation of opsonizing IgG rather than returning it to the circulation. Recombinant protein corresponding to aa22-301 from mouse FCRN, fused to 6-His-tag at C-terminal, expressed in murine myeloma cell line, NS0. Molecular Weight: ~32.3kD Biological Activity: Measured by its binding ability in a functional ELISA. When recombinant mouse FCRN is immobilized at 0.5ug/ml (100ul/well), the concentration of human IgG that produces 50% of the optimal binding response is found to be ~2-10ug/ml. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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