Androgen Receptor (AR) Recombinant, Human
Catalog No : USB-153465
412.66€
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| Product name | Androgen Receptor (AR) Recombinant, Human | ||
|---|---|---|---|
| Catalog No | USB-153465 | ||
| Supplier’s Catalog No | 153465 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant Human from E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 31.4 | ||
| Storage | -70°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥95% | ||
| Form | Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose. Reconstitute with 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥95% | ||
| Description | Recombinant protein corresponding to Pro15-Ser246 with an N-Terminal His and GST Tag from human, expressed in E. coli Swiss/UniProt Accession: P10275 Amino Acid Sequence: PSKTYRGAFQ NLFQSVREVI QNPGPRHPEA ASAAPPGASL LLLQQQQQQQ QQQQQQQQQQ QQQQQQETSP RQQQQQQGED GSPQAHRRGP TGYLVLDEEQ QPSQPQSALE CHPERGCVPE PGAAVAASKG LPQQLPAPPD EDDSAAPSTL SLLGPTFPGL SSCSADLKDI LSEASTMQLL QQQQQEAVSE GSSSGRAREA SGAPTSSKDN YLGGTSTISD NAKELCKAVS VS Predicted Isoelectric Point: 5.1 Predicted Molecular Weight: 31.4kD Accurate Molecular Mass: 36kD as determined by SDS-PAGE under reducing conditions Accurate Molecular Mass: 26kD as determined by SDS-PAGE reducing conditions Note: The possible reasons that the actual band size differs from the predicted are as follows: 1. Splice variants: Alternative splicing may create different sized proteins from the same gene 2. Relative charge: The composition of amino acids may affect the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. Subcellular Location: Nucleus, Cytoplasm Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions. | ||
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