MRPL13, Recombinant, Human, aa1-178, His-Tag (Mitochondrial Ribosomal Protein L13, L13, L13A, L13mt, RPL13, RPML13)

Catalog No : USB-137124
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Product name MRPL13, Recombinant, Human, aa1-178, His-Tag (Mitochondrial Ribosomal Protein L13, L13, L13A, L13mt, RPL13, RPML13)
Catalog No USB-137124
Supplier’s Catalog No 137124
Supplier US Biologicals
Source antigen Recombinant, E.coli
Reactivity
Cross reactivity
Applications
Molecular weight 23.1
Storage -20°C
Other names
Grade Highly Purified
Purity ~85% (SDS-PAGE)
Form Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.4M urea, 10% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity ~85% (SDS-PAGE)
Description Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL13 is a 39S subunit protein. Source: Recombinant protein corresponding to human MRPL13, fused to His-tag at N-terminal expressed in E.coli. Molecular Weight: ~23.1kD (201aa) AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSSFSRA PQQWATFARI WYLLDGKMQP PGKLAAMASI RLQGLHKPVY HALSDCGDHV VIMNTRHIAF SGNKWEQKVY SSHTGYPGGF RQVTAAQLHL RDPVAIVKLA IYGMLPKNLH RRTMMERLHL FPDEYIPEDI LKNLVEELPQ PRKIPKRLDE YTQEEIDAFP RLWTPPEDYR L Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.