Nudix-type Motif 21, aa1-227, Recombinant, Human (Nucleoside Diphosphate Linked Moiety X Motif 21, Nudix Motif 21, NUDT21, CFIM25, Cleavage and Polyadenylation Specificity Factor Subunit 5, CPSF5, Cleavage and Polyadenylation Specificity Factor 25kD Subunit, CPSF 25kD Subunit, CPSF25, Pre-mRNA Cleavage Factor Im 25kD Subunit)
Catalog No : USB-N7400-25B
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| Product name | Nudix-type Motif 21, aa1-227, Recombinant, Human (Nucleoside Diphosphate Linked Moiety X Motif 21, Nudix Motif 21, NUDT21, CFIM25, Cleavage and Polyadenylation Specificity Factor Subunit 5, CPSF5, Cleavage and Polyadenylation Specificity Factor 25kD Subunit, CPSF 25kD Subunit, CPSF25, Pre-mRNA Cleavage Factor Im 25kD Subunit) | ||
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| Catalog No | USB-N7400-25B | ||
| Supplier’s Catalog No | N7400-25B | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 28.3 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~90% (SDS-PAGE) | ||
| Form | Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 2mM DTT, 20% glycerol. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~90% (SDS-PAGE) | ||
| Description | NUDT21, also known as CPSF5 or CFIm25, is a member of the Nudix hydrolase family of pyrophosphatases. This protein localizes to the paraspeckles and forms a heterodimer with CPSF6 or CPSF7 to comprise the CFIm (mammalian cleavage factor I) complex. NUDT21 is the smaller subunit of the complex and is present in all heterodimer combinations. NUDT21 plays an important role in pre-mRNA 3' cleavage and polyadenylation processing. Source: Recombinant corresponding to human NUDT21, fused to His-tag at N-terminus, expressed in E. coli. Molecular Weight: ~28.3kD Sequences of Amino Acids: MGSSHHHHHH SSGLVPRGSH MSVVPPNRSQ TGWPRGVTQF GNKYIQQTKP LTLERTINLY PLTNYTFGTK EPLYEKDSSV AARFQRMREE FDKIGMRRTV EGVLIVHEHR LPHVLLLQLG TTFFKLPGGE LNPGEDEVEG LKRLMTEILG RQDGVLQDWV IDDCIGNWWR PNFEPPQYPY IPAHITKPKE HKKLFLVQLQ EKALFAVPKN YKLVAAPLFE LYDNAPGYGP IISSLPQLLS RFNFIYN Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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