Lin28B, Recombinant, Human, aa1-250, His-Tag (Protein Lin-28 Homolog B, CSDD2)

Catalog No : USB-138686
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Product name Lin28B, Recombinant, Human, aa1-250, His-Tag (Protein Lin-28 Homolog B, CSDD2)
Catalog No USB-138686
Supplier’s Catalog No 138686
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 29.5
Storage -20°C
Other names
Grade Purified
Purity ~85% (SDS-PAGE)
Form Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.2M sodium chloride, 2mM DTT, 10% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity ~85% (SDS-PAGE)
Description Lin28B belongs to the lin-28 family. LIN28 protein is a microRNA-binding protein that binds to and enhances the translation of the IGF-2 (insulin-like growth factor 2) mRNA. Lin28B is acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Source: Recombinant protein corresponding to aa1-250 from human Lin28B at N-terminal, fused to His-tag expressed in E.coli. Molecular Weight: ~29.5kD (273aa) AA Sequence: MGSSHHHHHH SSGLVPRGSH MGS KLFMEGFRSL KEGEPVEFTF KKSSKGLESI RVTGPGGSPC LGSERRPKGK TLQKRKPKGD RCYNCGGLDH HAKECSLPPQ PKKCHYCQSI MAEGGAS KGGGEEPGKL PEPAEEESQV LRGTGHCKWF NVRMGFGFIS MINREGSPLD IPVDVFVHQS MHMVANCPHK NVAQPPASSQ GRQEAESQPC TSTLPREVGG GHGCTSPPFP QEARAEISER SGRSPQEASS TKSSIAPEEQ SKKGPSVQKR KKT Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.