Imugex

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Categories: Peptides & Proteins / Proteins

Product name			SEPW1, Recombinant, Human, aa1-87, His-Tag (Selenoprotein W)
Catalog No			USB-218547
Supplier’s Catalog No			218547
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			11.8

Storage			-20°C
Other names
Grade			Highly Purified
Purity			~95% (SDS-PAGE)
Form			Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15M sodium chloride, 1mM DTT, 20% glycerol.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~95% (SDS-PAGE)
Description			SEPW1 is a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the uGA codon that normally signals translation termination. The 3' uTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of uGA as a Sec codon rather than as a stop signal. This protein shows highest expression in skeletal muscle and heart, and may be involved in oxidation-reduction reactions. A retroprocessed pseudogene, SEPW1P, has been identified and mapped to chromosome 1p35-34. Source: Recombinant protein corresponding to aa1-87 from human SEPW1, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~11.8kD (110aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.