Secretin, alpha, Recombinant, Human (GST)
Catalog No : USB-S0625-15
433.35€
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| Product name | Secretin, alpha, Recombinant, Human (GST) | ||
|---|---|---|---|
| Catalog No | USB-S0625-15 | ||
| Supplier’s Catalog No | S0625-15 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 52.4 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥ 90% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | ||
| Form | Supplied as a liquid in 50mM Tris buffer, pH 8.0 and 10mM glutathione. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥ 90% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | ||
| Description | Erythrocyte spectrin is the main component of the red cell membrane skeleton responsible for the shape and physical properties of red cells. Spectrin is composed of 2 subunits alpha and beta (280 and 246kD respectively). The human erithroid a-spectrin (2418 amino acids) consists of 22 repeating segments of about 106 amino acids in length. This protein was demonstrated to be a specific substrate for ubiquitina tion in vitro and in vivo. The main ubiquitination site on human a-spectrin was identified in the lysine 27 of the repeating segment 17 by sitedirected mutagenesis. Cell-free experiments using radiolabeled, biotinylated or native ubiquitin and cellular lysates from rabbit retyculocytes, human erythrocytes or k562 cells, showed that only monoubiquitination occurs at this site. Description: Recombinant fusion protein: Glutathione-S-Transferase (GST) and a fragment of human a-spectrin (from Lysine 1601 to Leucine 1818) containing repeat 17 and the ubiquitination target lysine 27. The total molecular weight of the fusion protein is 52.4kD. Application: Substrate for cell-free ubiquitination assays. Stability: rGST-alpha Spectrin although stable at 140C for 1 week, should be stored desiccated below -180C. Please prevent freeze-thaw cycles. | ||
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