PLTP, Recombinant, Mouse (Phospholipid Transfer Protein)
Catalog No : USB-P4278-01
705.77€
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| Product name | PLTP, Recombinant, Mouse (Phospholipid Transfer Protein) | ||
|---|---|---|---|
| Catalog No | USB-P4278-01 | ||
| Supplier’s Catalog No | P4278-01 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, Mouse myeloma cell line, NS0-derived | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥95% (SDS-PAGE). Endotoxin: ≤1.0 EU/1ug (LAL). | ||
| Form | Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 50ug of BSA per ug of protein. Reconstitute with PBS. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥95% (SDS-PAGE). Endotoxin: ≤1.0 EU/1ug (LAL). | ||
| Description | Mouse PLTP Phospholipid transfer protein (PLTP; also lipid transfer protein II) is an 80kD, secreted glycoprotein, that belongs to the BPI/LBP/Plunc superfamily, BPI/LBP family of molecules (1). It is synthesized as a precursor with a 17aa signal sequence and a 476aa mature chain (SwissProt # P55065). The mature chain contains two BPI/LBP/CETP domains (aa21-239 and 263-463) and seven potential sites for N-linked glycosylation. Mature mouse PLTP shares 83% aa sequence identity with mature human PLTP (3). PLTP is expressed by a wide variety of tissues and is secreted by macrophages. Northern blot analysis shows the order of PLTP mRNA expression as follows: lung > adipose tissue, placenta, testis > brain > muscle, heart, liver (2). PLTP is a multifunctional, extracellular lipid transfer protein that plays a major role in lipoprotein metabolism and atherosclerosis (4-6). In addition to phospholipids, PLTP transfers several amphipatic compounds, including unesterified cholesterol, lipopolysaccharides, diacylglycerides, and alpha-tocopherol, the main isomer of vitamin E (4). According to several studies, PLTP not only affects plasma lipid and lipoprotein profiles, but also modulates cell physiology (4, 7-11). In mice, systemic PLTP deficiency proves to be atheroprotective, whereas PLTP overexpression is proatherogenic (4, 6). However, macrophage PLTP is shown to be atheroprotective (4, 6). Source: A DNA sequence encoding the mature mouse PLTP (Glu18-Ala493; AAH03782) was fused with a 6X-His tag at the C-terminus and expressed in a mouse myeloma cell line, NS0. Molecular Mass: Based on N-terminal amino acid sequencing, the recombinant mouse PLTP starts at Glu 18 and has a calculated molecular mass of approximately 53.5kD. The recombinant mouse PLTP migrates as an approximately 70-85kD protein in SDS-PAGE under reducing conditions. Activity: Measured by its ability to inhibit LPS-induced TNF-alpha secretion by Raw264.7 cells. Storage and Stability: 12 months from date of receipt, 20 to 70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, 20 to 70°C under sterile conditions after reconstitution. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. | ||
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