Folate Binding Protein, Bovine

Catalog No : USB-F5750-03
925.31€
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Product name Folate Binding Protein, Bovine
Catalog No USB-F5750-03
Supplier’s Catalog No F5750-03
Supplier US Biologicals
Source antigen Bovine Milk
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -20°C
Other names
Grade Highly Purified
Purity ≥95% (HPLC)
Form Supplied as a liquid in PBS, pH 7.4. No preservative added.
Reactivity life 12 months
Note For reserch purpose only
Purity ≥95% (HPLC)
Description Folate Binding Protein, has a high affinity for folic acid and for several reduced folic acid derivatives and mediates delivery of 5 methyltetrahydrofolate to the interior of cells. Membrane bound and soluble forms of a high affinity folate binding protein have been found in kidney, placenta, serum, milk, and in several cell lines. The 2 forms have similar binding characteristics for folates, are immunologically crossreactive, and, based upon limited amino acid sequence data, are nearly identical. There may be a precursor product relationship between the membrane and soluble forms, the membrane form having additional amino acid residues and greater molecular weight. The membrane form has been shown to mediate the transport of folate in cells grown in physiologic concentrations of folate. A function for the soluble form, which is found in serum, milk, and urine, has not been identified. Protein content (mg/ml): As reported (indirect value calculated using the measured activity in ug/ml and the binding capacity in ug folic acid/mg folate binding protein). Note: Concentration value is lot-specific Activity (ug folic acid per mg protein): As reported (Lot-specific) Applications: Suitable for use in ELISA. Other applications not tested Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.