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Categories: Peptides & Proteins / Proteins

Product name			Haptoglobin beta Chain, Recombinant, Human, Western Blot Control (HP)
Catalog No			USB-H1820-02D
Supplier’s Catalog No			H1820-02D
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			-20°C
Other names
Grade			Purified
Purity			Purified (SDS-PAGE)
Form			Supplied as a liquid in 25mM Tris-HCl, pH6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue, 2.5% glycerol.
Reactivity life			6 months
Note			For reserch purpose only
Purity			Purified (SDS-PAGE)
Description			Haptoglobin is a glycoprotein secreted in the blood plasma that binds free haemoglobin thereby inhibiting its oxidative activity and preventing iron loss following haemolysis. Haptoglobin also regulates the activity of many cell types of the immune system and is an extracellular chaperone. Haptoglobin is produced mostly by hepatocytes but also by other tissues: e.g. skin, lung and kidney. Haptoglobin is a tetramer of two alpha and two beta chains, connected by disulfide bridges. The chains originate from a common precursor protein, which is proteolytically cleaved during protein synthesis. Source: Recombinant corresponding to aa162-406 from mature human haptoglobin beta chain, fused to 6x His-tag at N-terminal, expressed in E. coli. Applications: Suitable for use in Western Blot as a control. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.