SIRT 3, Recombinant, Human, GST-Tag (Sirtuin 3, Sir2-related Protein Type 3, Sir2-like 3)
Catalog No : USB-S1013-88F2
697.72€
0.00€
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| Product name | SIRT 3, Recombinant, Human, GST-Tag (Sirtuin 3, Sir2-related Protein Type 3, Sir2-like 3) | ||
|---|---|---|---|
| Catalog No | USB-S1013-88F2 | ||
| Supplier’s Catalog No | S1013-88F2 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 59 | ||
| Storage | -70°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥ 90% by SDS-PAGE | ||
| Form | Supplied as a liquid in 50mM Tris-HCl, pH 8.0, 138mM NaCl, 20mM glutathione and 10% glycerol. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥ 90% by SDS-PAGE | ||
| Description | Human Sirtuin 3 (GenBank Accession No. NM_012239), amino acids 102- 399 with N-terminal GST tag, expressed in E. coli expression system. Specific Activity: 6 U/ug. One U =1 pmol/min Assay Condition: 25mM Tris/Cl, pH 8.0, 137mM NaCl, 2.7mM KCl, 1mM MgCl2 and 0.1mg/ml BSA, 30uM BPS HDAC substrate, 200uM NAD+ and 20ng/ul Sirtuin 3. Incubation Condition: 30 min at 30ºC Application: Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. | ||
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