SHH, Recombinant, Mouse (Sonic Hedgehog Protein, HHG-1, Hhg1)
Catalog No : USB-S1012-96N
526.45€
0.00€
Shipping cost plus VAT not included , delivery in 7-14 business days
| Product name | SHH, Recombinant, Mouse (Sonic Hedgehog Protein, HHG-1, Hhg1) | ||
|---|---|---|---|
| Catalog No | USB-S1012-96N | ||
| Supplier’s Catalog No | S1012-96N | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 19.8 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~97% (SDS-PAGE, HPLC). Endotoxin: <0.01ng/ug | ||
| Form | Supplied as a lyophilied powder from 10mM sodium phosphate buffer, pH 7.5. Reconstitute with sterile ddH2O to 0.1-0.5mg/ml. Add 0.1% BSA or HSA for long term storage. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~97% (SDS-PAGE, HPLC). Endotoxin: <0.01ng/ug | ||
| Description | Recombinant Mouse Sonic Hedgehog is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Mouse Sonic Hedgehog is 99% homologous to the human gene. Cysteine at position 25 has been substituted with Ile. Mouse SHH is a non-glycosylated polypeptide consisting of 176aa with a molecular weight of 19.8kD. Recombinant corresponding to mouse Sonic Hedgehog expressed in E. coli. Amino Acid Sequence: MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG Biological Activity: Mouse SHH is fully biologically active when compared to standard. Determined by its’ ability to induce alkaline phosphatase production by C3H10T1/2 fibroblasts, with an expected ED50 in the range of 0.03-0.30ug/ml. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with ddH2O, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
Product Details
© 2020 Imugex All Rights Reserved