Sonic HedgeHog, Recombinant, Human (Shh)
Catalog No : USB-361220
477.02€
0.00€
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| Product name | Sonic HedgeHog, Recombinant, Human (Shh) | ||
|---|---|---|---|
| Catalog No | USB-361220 | ||
| Supplier’s Catalog No | 361220 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 20 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~98% (SDS-PAGE, HPLC) | ||
| Form | Supplied as a lyophilized powder in 20mM PB, pH 7.4, 150mM sodium chloride. Reconstitute with sterile dH2O, 0.1% BSA to 0.1-1mg/ml. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~98% (SDS-PAGE, HPLC) | ||
| Description | Sonic hedgehog (SHH) is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology and all signal via the same receptors. Source: Recombinant protein corresponding to human Sonic HedgeHog, a single non-glycosylated polypeptide chain containing 176aa, expressed in E. coli. Molecular Weight: ~20kD (176aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Biological Activity: Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.8-1ug/ml, corresponding to a specific activity of ＞1x10e3IU/mg. Endotoxin: <1EU/ug (LAL) AA Sequence: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile dH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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