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Categories: Peptides & Proteins / Proteins

Product name			Desert Hedgehog, Recombinant, Human (Desert Hedgehog Protein, DHH)
Catalog No			USB-499927
Supplier’s Catalog No			499927
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			20

Storage			-20°C
Other names
Grade			Highly Purified
Purity			~95% (SDS-PAGE)
Form			Supplied as a lyophilized powder in PBS, 0.2mM DTT. Reconstitute with sterile PBS to ~0.5mg/ml.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~95% (SDS-PAGE)
Description			The Desert Hedgehog protein is one of the three Hedgehog family proteins that is highly conserved and plays a role in many developmental processes in animals. DHH become biologically active following its autocatalytic cleavage. There is a high degree of residue homology between Sonic Hedgehog (SHH), Indian Hedgehog (IHH), and DHH. Despite their individual levels of expression and unique biological roles, the Hedgehog family members all use the same signalling pathway, and therefore, may be used interchangeably for some experimental purposes. Source: Recombinant protein corresponding to human DHH expressed in E. coli. Molecular Weight: ~20kD Biological Activity: Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 15-45ug/mL. Endotoxin: <1EU/ug (LAL) Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.