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Categories: Peptides & Proteins / Proteins

Product name			Thioredoxin-1, aa2-109, Recombinant, E. coli (Trx-1, trxA, b3781, dasC, fipA, JW5856, tsnC)
Catalog No			USB-T5010-10E
Supplier’s Catalog No			T5010-10E
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			12.9

Storage			-20°C
Other names
Grade			Highly Purified
Purity			~ 85% (SDS-PAGE). Purified by using conventional chromatography techniques.
Form			Supplied as a liquid in 20mM Tris HCl, pH 8.0, 1mM DTT, 10% glycerol.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~ 85% (SDS-PAGE). Purified by using conventional chromatography techniques.
Description			Thioredoxin 1 (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant E. coli TXN protein, fused to His-tag at N-terminus, was expressed in E. coli. Source: Recombinant corresponding to aa2-109 of human Thioredoxin 1 6x His tagged expressed in E. coli. AA Sequence: MHHHHHHMGS DKIIHLTDDS FDTDVLKADG AILVDFWAEW CGPCKMIAPI LDEIADEYQG KLTVAKLNID QNPGTAPKYG IRGIPTLLLF KNGEVAATKV GALSKGQLKE FLDANLAGS Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.